The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 23, 2025
Filed:
Nov. 09, 2021
Jiangnan University, Jiangsu, CN;
Chuanlai Xu, Jiangsu, CN;
Jingjing Yao, Jiangsu, CN;
Hua Kuang, Jiangsu, CN;
Liguang Xu, Jiangsu, CN;
Maozhong Sun, Jiangsu, CN;
Xiaoling Wu, Jiangsu, CN;
Liqiang Liu, Jiangsu, CN;
Wei MA, Jiangsu, CN;
Jianping Zhu, Jiangsu, CN;
Changlong Hao, Jiangsu, CN;
Shanshan Song, Jiangsu, CN;
Yongming Hu, Jiangsu, CN;
Aihong Wu, Jiangsu, CN;
Lingling Guo, Jiangsu, CN;
Xinxin Xu, Jiangsu, CN;
JIANGNAN UNIVERSITY, Jiangsu, CN;
Abstract
The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.