The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 2019
Filed:
Nov. 10, 2015
Lallemand Hungary Liquidity Management Llc, Budapest, HU;
Stellenbosch University, Stellenbosch, ZA;
Elena Brevnova, Labanon, NH (US);
John E. McBride, Lyme, NH (US);
Erin Wiswall, Danbury, NH (US);
Kevin S. Wenger, Hanover, NH (US);
Nicky Caiazza, Rancho Santa Fe, CA (US);
Heidi Lau, Lebanon, NH (US);
Aaron Argyros, White River Junction, VT (US);
Frank Agbogbo, Lebanon, NH (US);
Charles F. Rice, Hopkinton, NH (US);
Trisha Barrett, Bradford, VT (US);
John S. Bardsley, Newport, NH (US);
Abigail Foster, South Strafford, VT (US);
Anne K. Warner, Lebanon, NH (US);
Mark Mellon, Grantham, NH (US);
Ryan Skinner, White River Junction, VT (US);
Indraneel Shikhare, Lebanon, NH (US);
Riaan Den Haan, Vierlanden, ZA;
Chhayal V. Gandhi, Lebanon, NH (US);
Alan Belcher, Nashua, NH (US);
Vineet B. Rajgarhia, Courbevoie, FR;
Allan C. Froehlich, Lebanon, NH (US);
Kristen M. Deleault, Canaan, NH (US);
Emily Stonehouse, Lebanon, NH (US);
Shital A. Tripathi, Berkley, CA (US);
Jennifer Gosselin, Lebanon, NH (US);
Yin-Ying Chiu, West Lebanon, NH (US);
Haowen Xu, Lebanon, NH (US);
Lallemand Hungary Liquidity Management LLC, Budapest, HU;
Stellenbosch University, Stellenbosch, ZA;
Abstract
The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.