Richard J Ridge

Title: Richard J Ridge: Innovator in Recombinant Polypeptide Production

Introduction

Richard J Ridge is a notable inventor based in Acton, MA (US), recognized for his contributions to the field of recombinant DNA technology. He holds a total of 8 patents, showcasing his innovative approach to the production, isolation, and purification of polypeptides.

Latest Patents

Among his latest patents, Ridge has developed a method for the isolation and purification of polypeptides expressed in host cells through recombinant DNA techniques. This method involves producing a fused polypeptide that includes a desired polypeptide linked to additional amino acids, which define a leader sequence with exploitable properties in purification. The hinge region of the polypeptide is cysteine-free and designed to expose a cleavage site to a selected endopeptidase, allowing for efficient isolation and cleavage in an appropriate aqueous environment.

Another significant patent involves a novel polypeptide that serves as a leader or trailer peptide moiety in recombinant DNA protein production techniques. This polypeptide is designed to have hydrophilic charged amino acid residues on one side and nonpolar amino acid residues on the other side of an amphiphilic helix. This design facilitates high-level expression and the spontaneous formation of inclusion bodies, which can be easily collected and purified by adjusting the ionic strength and/or pH of the media.

Career Highlights

Throughout his career, Richard J Ridge has worked with several prominent companies, including Creative Biomolecules, Inc. and Stryker Corporation. His work has significantly advanced the field of biotechnology, particularly in the area of protein production.

Collaborations

Ridge has collaborated with notable individuals in his field, including James S Huston and Charles M Cohen. These collaborations have contributed to the development of innovative solutions in recombinant DNA technology.

Conclusion

Richard J Ridge's contributions to the field of recombinant polypeptide production have made a lasting impact on biotechnology. His innovative patents and collaborations highlight his role as a leading inventor in this important area of research.