The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 12, 2006
Filed:
Feb. 04, 1993
James S. Huston, Newton, MA (US);
Marc F. Charette, West Roxbury, MA (US);
Charles M. Cohen, Medway, MA (US);
Roberto Crea, Boston, MA (US);
Peter C. Keck, Millbury, MA (US);
Hermann Oppermann, Medway, MA (US);
David C. Rueger, West Roxbury, MA (US);
Richard J. Ridge, Acton, MA (US);
James S. Huston, Newton, MA (US);
Marc F. Charette, West Roxbury, MA (US);
Charles M. Cohen, Medway, MA (US);
Roberto Crea, Boston, MA (US);
Peter C. Keck, Millbury, MA (US);
Hermann Oppermann, Medway, MA (US);
David C. Rueger, West Roxbury, MA (US);
Richard J. Ridge, Acton, MA (US);
Micromet AG, Munich, DE;
Abstract
Disclosed is a method for the isolation and purification of polypeptides expressed in host cells by recombinant DNA techniques. A fused polypeptide is produced containing a desired polypeptide fused to additional amino acids. The additional amino acids define a leader sequence having properties exploitable in purification, a hinge region, and a cleavage site. The hinge region is cysteine-free and has a secondary structure which serves to expose the cleavage site to a selected endopeptidase. The method of the invention involves the production of a fused polypeptide which may be efficiently isolated by exploiting the properties of the leader sequence, and then efficiently cleaved at the cleavage site in an appropriate aqueous environment by virtue of the influence of the hinge on the cleavage agent/cleavage site reaction and other properties of the fused polypeptide.