Harold Ray Lee

Title: Harold Ray Lee: Innovator in Nucleotide Sequencing Technologies

Introduction

Harold Ray Lee is a prominent inventor based in Sunnyvale, CA, known for his significant contributions to the field of biotechnology. He holds a total of 6 patents that focus on enhancing DNA sequencing technologies. His work has paved the way for advancements in single molecule sequencing, which is crucial for various applications in genomics.

Latest Patents

One of Harold Ray Lee's latest patents is titled "Recombinant polymerases for improved single molecule sequencing." This invention provides compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features. These modifications confer enhanced properties upon the polymerase, such as improved metal ion coordination, reduced exonuclease activity, and increased accuracy and speed in sequencing. Additionally, the patent includes nucleic acids that encode these polymerases and methods for utilizing them in DNA sequencing.

Another notable patent is "Step-wise nucleic acid sequencing with catalytic and non-catalytic metals." This application relates to methods and systems for nucleotide sequencing that involve polymerase reactions with both catalytic and non-catalytic divalent metal ions. The polymerase/template/primer complexes are immobilized on a substrate and exposed to a solution containing a non-catalytic metal and labeled nucleotides. This innovative approach allows for the observation of sequestered cognate nucleotides, leading to more efficient incorporation and sequencing processes.

Career Highlights

Harold Ray Lee has made significant strides in the biotechnology sector, particularly through his work at Pacific Biosciences of California, Inc. His inventions have contributed to the development of advanced sequencing technologies that are widely used in research and clinical applications.

Collaborations

Throughout his career, Harold has collaborated with notable colleagues, including Keith P Bjornson and Fred C Christians. These partnerships have fostered innovation and have been instrumental in advancing the field of nucleotide sequencing.

Conclusion

Harold Ray Lee's contributions to biotechnology, particularly in the realm of DNA sequencing, have established him as a key figure in the industry. His innovative patents and collaborative efforts continue to influence advancements in genomic research and applications.