The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 16, 2013
Filed:
Sep. 30, 2010
Sonya Clark, Oakland, CA (US);
Arek Bibillo, Cupertino, CA (US);
Paul Peluso, San Carlos, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Molly He, Palo Alto, CA (US);
Insil Park, Fremont, CA (US);
Harold Lee, Sunnyvale, CA (US);
Keith Bjornson, Newark, CA (US);
Lei Jia, Palo Alto, CA (US);
Robin Emig, Belmont, CA (US);
Sonya Clark, Oakland, CA (US);
Arek Bibillo, Cupertino, CA (US);
Paul Peluso, San Carlos, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Molly He, Palo Alto, CA (US);
Insil Park, Fremont, CA (US);
Harold Lee, Sunnyvale, CA (US);
Keith Bjornson, Newark, CA (US);
Lei Jia, Palo Alto, CA (US);
Robin Emig, Belmont, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.