The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 04, 2012
Filed:
Mar. 30, 2009
Sonya Clark, Oakland, CA (US);
Arek Bibillo, Cupertino, CA (US);
Paul Peluso, San Carlos, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Molly He, Palo Alto, CA (US);
Insil Park, Fremont, CA (US);
Harold Lee, Sunnyvale, CA (US);
Keith Bjornson, Newark, CA (US);
Lei Jia, Palo Alto, CA (US);
Robin Emig, Belmont, CA (US);
Sonya Clark, Oakland, CA (US);
Arek Bibillo, Cupertino, CA (US);
Paul Peluso, San Carlos, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Molly He, Palo Alto, CA (US);
Insil Park, Fremont, CA (US);
Harold Lee, Sunnyvale, CA (US);
Keith Bjornson, Newark, CA (US);
Lei Jia, Palo Alto, CA (US);
Robin Emig, Belmont, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.