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Title: Innovations and Contributions of Abraham Johannes De Smit
Introduction
Abraham Johannes De Smit is a prominent inventor based in Dronten, Netherlands, known for his significant contributions in the field of recombinant protein production. With a portfolio of two patents, he has made noteworthy advancements in methodologies that enhance protein expression in baculovirus vector systems.
Latest Patents
One of his latest patents is titled "E2 subunit vaccine comprising recombinant pestivirus E2 protein." This invention relates to a method designed to increase protein expression in baculovirus expression systems. The patented method includes steps to produce a recombinant protein in insect cell cultures. It specifies selecting a recombinant baculovirus expressing the desired protein, cultivating insect cells in growth medium, and infecting the cells with a baculovirus inoculum at defined cell densities and multiplicities of infection (m.o.i). Notably, the invention aims to achieve a final concentration of recombinant pestivirus E2 protein or its fragments in the growth medium of at least 100 µg/ml.
In addition, his patent titled "Protein expression in baculovirus vector expression systems" offers a similar methodology that focuses on the production of recombinant follicle-stimulating hormone (FSH) and its subunits, ensuring that the concentration at harvest reaches at least 15 µg/ml in the growth medium.
Career Highlights
Abraham has played pivotal roles in various research endeavors throughout his career. He worked for organizations such as Stichting Instituut Voor Dierhouderij En Diergezondheid, where he focused on advancing scientific knowledge related to animal health and production. His innovative approach to protein expression has been a valuable asset in research and development settings.
Collaborations
Throughout his career, Abraham has collaborated with talented professionals in his field, notably working alongside Dietmar Kretzdorn and Dirk Franciscus Marinus Van De Wiel. These collaborations have facilitated the exchange of ideas and innovations, further enhancing the impact of their collective work in recombinant protein research.
Conclusion
Abraham Johannes De Smit stands out as an accomplished inventor with a focus on improving protein production methods in biotechnological applications. His patents illustrate his commitment to advancing scientific knowledge and practical applications in the field, solidifying his reputation as a key player in innovation and biotechnology.