The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 29, 2003
Filed:
Jun. 16, 2000
Dietmar Kretzdorn, Leichlingen, DE;
Dirk Franciscus Marinus van de Wiel, Hardewijk, NL;
Abraham Johannes de Smit, Dronten, NL;
Robertus Jacobus Maria Moormann, Dronten, NL;
Erik Kees Hamann, Lelystad, NL;
Other;
Abstract
The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×10 to 5×10 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 &mgr;g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, &agr;-units an/or &bgr;-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &mgr;l.