The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2005
Filed:
Feb. 27, 2003
Dietmar Kretzdorn, Leichlingen, DE;
Dirk Franciscus Marinus Van DE Wiel, Hardewijk, NL;
Abraham Johannes DE Smit, Dronten, NL;
Robertus Jacobus Maria Moormann, Dronten, NL;
Erik Kees Hamann, Lelystad, NL;
Dietmar Kretzdorn, Leichlingen, DE;
Dirk Franciscus Marinus van de Wiel, Hardewijk, NL;
Abraham Johannes de Smit, Dronten, NL;
Robertus Jacobus Maria Moormann, Dronten, NL;
Erik Kees Hamann, Lelystad, NL;
Stichting Instituut voor Dierhouderij en Diergezondheid, Lelystad, NL;
Bayer AG, Leverkussen, NL;
Abstract
The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×10to 5×10cells/ml with an m.o.i of <0.01. The invention also provides a method to produce recombinant pestivirus E2 or Eprotein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 μg/ml. The invention also provides a method of producing recombinant FSH, α-units and/or β-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 μ/ml.