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Bayer Aktiengesellschaft

Stichting Instituut Voor Dierhouderij En Diergezondheid

Abraham Johannes De Smit

Robertus Jacobus Maria Moormann

Dirk Franciscus Marinus Van De Wiel

Dietmar Kretzdorn

Erik Kees Hamann

The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1&#xd7;10to 5&#xd7;10cells/ml with an m.o.i of &#x3c;0.01. The invention also provides a method to produce recombinant pestivirus E2 or Eprotein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 &#x3bc;g/ml. The invention also provides a method of producing recombinant FSH, &#x3b1;-units and/or &#x3b2;-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &#x3bc;/ml.

E2 subunit vaccine comprising recombinant pestivirus E2 protein

The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1&times;10 to 5&times;10 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 &mgr;g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, &agr;-units an/or &bgr;-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &mgr;l.

Protein expression in baculovirus vector expression systems

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Dronten, Netherlands

Abraham Johannes De Smit