The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 2012
Filed:
Apr. 06, 2009
Yijie Gao, Brookline, MA (US);
Nicole M. Piche, Waltham, MA (US);
Mei Geng, Bedford, MA (US);
Stephen H. Herrmann, Stowe, MA (US);
Xiaotian Zhong, Wayland, MA (US);
Ronald Kriz, Northborough, MA (US);
Zhijian LU, Bedford, MA (US);
Yijie Gao, Brookline, MA (US);
Nicole M. Piche, Waltham, MA (US);
Mei Geng, Bedford, MA (US);
Stephen H. Herrmann, Stowe, MA (US);
Xiaotian Zhong, Wayland, MA (US);
Ronald Kriz, Northborough, MA (US);
Zhijian Lu, Bedford, MA (US);
Wyeth LLC, Madison, NJ (US);
Abstract
The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XB1- or ATF6-mediated UPR pathway.