The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 10, 1998
Filed:
Mar. 05, 1997
Donald G Comb, Manchester, MA (US);
Francine B Perler, Brookline, MA (US);
William E Jack, Wenham, MA (US);
Ming-Qun Xu, Hamilton, MA (US);
Robert A Hodges, Norcross, GA (US);
Christopher J Noren, Boxford, MA (US);
Shaorong S Chong, Beverly, MA (US);
Eric Adam, Beverly, MA (US);
Maurice Southworth, Beverly, MA (US);
New England Biolabs, Inc., Beverly, MA (US);
Abstract
The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into or adjacent a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity. The CIVPS may be used in a number of applications including purification of the target protein in a one-step protocol.