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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12428628 B1

PDFFull Text
Date of Patent:
Sep. 30, 2025

Filed:

Jun. 27, 2024

Recombinant production of steviol glycosides

Applicant:

Danstar Ferment Ag, Schweiz, CH;

Inventors:

Ganesh M. Kishore, Saint Louis, MO (US);

Michael Motion, San Francisco, CA (US);

Paula M. Hicks, Bend, OR (US);

Jorgen Hansen, Frederiksberg, DK;

Jens Houghton Larsen, Birkerod, DK;

Esben Halkjaer Hansen, Frederiksberg, DK;

Michael Dalgaard Mikkelsen, Vaerlose, DK;

Sabina Tavares, San Francisco, CA (US);

Charlotte Blom, San Francisco, CA (US);

Assignee:

Danstar Ferment AG, Schweiz, CH;

Attorney:

McDonnell Boehnen Hulbert & Berghoff LLP

Primary Examiner:

Weihua Fan

Int. Cl.
CPC ...
C12N 9/10 (2006.01); A23L 2/60 (2006.01); A23L 27/30 (2016.01); C07H 15/256 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01); C12N 15/81 (2006.01); C12N 15/82 (2006.01); C12P 7/42 (2006.01); C12P 15/00 (2006.01); C12P 19/56 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1051 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07H 15/256 (2013.01); C12N 9/0006 (2013.01); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/1077 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12N 15/8243 (2013.01); C12N 15/8245 (2013.01); C12P 7/42 (2013.01); C12P 15/00 (2013.01); C12P 19/56 (2013.01); C12Y 101/01088 (2013.01); C12Y 106/02004 (2013.01); C12Y 114/13078 (2013.01); C12Y 114/13079 (2013.01); C12Y 204/01126 (2013.01); C12Y 205/01029 (2013.01); C12Y 402/03019 (2013.01); C12Y 505/01012 (2013.01); Y02P 20/52 (2015.11);
Abstract

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

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