The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 01, 2025

Filed:

Jul. 03, 2024
Applicant:

Jiangsu University, Zhenjiang, CN;

Inventors:

Huanhuan Li, Zhenjiang, CN;

Qiyi Bei, Zhenjiang, CN;

Zhixiong Qi, Zhenjiang, CN;

Quansheng Chen, Zhenjiang, CN;

Yiyong Yan, Zhenjiang, CN;

Hongzhen Ma, Zhenjiang, CN;

Xin Zhang, Zhenjiang, CN;

Qin Ouyang, Zhenjiang, CN;

Assignee:

JIANGSU UNIVERSITY, Zhenjiang, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/18 (2006.01); C12Q 1/00 (2006.01); C12Q 1/04 (2006.01); C12Q 1/689 (2018.01); G01N 1/38 (2006.01); G01N 21/64 (2006.01); G01N 33/12 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6428 (2013.01); G01N 1/38 (2013.01); G01N 33/12 (2013.01);
Abstract

A method for determining ciprofloxacin in a meat product based on an enzyme-linked upconversion fluorescence and potassium titanium oxalate system is provided. An enzyme-linked upconversion nanoparticle solution is mixed with an aptamer-bound magnetized polydopamine solution to obtain a fluorescence-ultraviolet dual-signal detection system. A fluorescence standard curve and a colorimetric standard curve for the ciprofloxacin content determination are created through the fluorescence-ultraviolet dual-signal detection system in combination with potassium titanium oxalate and hydrogen peroxide. A fluorescence intensity signal characteristic value and an ultraviolet absorbance signal characteristic value of a meat sample solution are determined. A ciprofloxacin content in the meat sample is calculated according to the fluorescence standard curve and the colorimetric standard curve.


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