Method for domesticating

Abstract

The present disclosure provides a method for domesticatingwhich is resistant to fermentation inhibitors, high temperature and high concentration of ethanol. The method comprises a primary domestication and a secondary domestication of the, wherein the primary domestication comprises the steps of replacing the fermented mash with fresh liquefied mash and continuing fermentation when the ethanol content in the fermented mash reaches a higher concentration (>12% (v/v)), the replacement is performed for at least 20 times, and the time interval of replacements is 20-30 hours; the secondary domestication comprises the steps of performing high-temperature domestication on the primary domesticated strain by a temperature gradient and replacing the fermented mash for multiple times, wherein the temperature is raised by 0.5° C.-2° C. every 18-24 times of replacement. The domesticatedin the present disclosure not only adapts to the fermentation conditions of factories, resists the fermentation substrate-inhibitors, but also maintains high production capacity of ethanol, and is still efficiently propagated and metabolized in a higher temperature environment, such that the ethanol fermentation level of enterprises can be improved without modifying process routes and conditions.