Company Filing History:
9 out of 336 
1 out of 832,680 Years Active: 2019-2025
Title: Innovations by Vladimir Potapov
Introduction
Vladimir Potapov is a notable inventor based in Auburndale, MA (US), recognized for his significant contributions to the field of biotechnology. He holds a total of 10 patents, showcasing his innovative approach to DNA assembly and manipulation.
Latest Patents
Among his latest patents is the "Ordered assembly of multiple DNA fragments," which provides a composition and its uses, including a kit. This composition features a synthetic self-complementary oligonucleotide with a double-stranded region and a loop, designed to include a binding sequence for PaqCI. The oligonucleotide also has unligatable 3' and 5′ ends that are resistant to cleavage by PaqCI. This innovative oligonucleotide composition is combined with PaqCI or its variants in a reaction mixture, which may also include a ligase, a deadenylase, a crowding molecule like PEG, and a repair enzyme such as Endo MS.
Another significant patent is for "Compositions and methods for improved in vitro assembly of polynucleotides." This invention enhances the ordered assembly of numerous fragments into a single large DNA molecule, improving both the frequency and fidelity of the assembled product. The method utilizes a computer system that integrates comprehensive ligation data to optimize synthetic overhangs or overhangs from restriction endonuclease cleavage. A novel restriction endonuclease that recognizes seven nucleotides and creates four-base overhangs is employed, along with variations in ligation preferences by different ligases to ensure precision in assembly reactions. This method has been successfully exemplified by the assembly of a viral genome from 52 fragments and a bacterial operon from the same number of fragments.
Career Highlights
Vladimir Potapov has worked with reputable organizations, including New England Biolabs, Inc. His experience in these companies has contributed to his expertise in the field of biotechnology and DNA manipulation.
Collaborations
He has collaborated with notable coworkers such as Jennifer Ong and Gregory Lohman, further enhancing his innovative projects and research.
Conclusion
Vladimir Potapov's contributions to biotechnology through his patents and collaborations highlight his role as a leading inventor in the field. His work continues to influence advancements in DNA assembly and manipulation.
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