The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2023
Filed:
Aug. 21, 2018
New England Biolabs, Inc., Ipswich, MA (US);
Kelly M. Zatopek, Wilmington, MA (US);
Vladimir Potapov, Auburndale, MA (US);
Jennifer Ong, Salem, MA (US);
Laurence Ettwiller, Beverly, MA (US);
Lixin Chen, Beverly, MA (US);
Thomas C. Evans, Jr., Topsfield, MA (US);
Andrew F. Gardner, Manchester, MA (US);
Other;
Abstract
Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.