Location History:
- Tsuruga, JP (2003 - 2013)
- Tokyo, JP (2013 - 2017)
Company Filing History:
6 out of 789 
2 out of 1,287 
2 out of 6 
Years Active: 2003-2017
Title: Shigeaki Nishii: Innovator in Protein Detection Technologies
Introduction
Shigeaki Nishii is a prominent inventor based in Tsuruga, Japan. He has made significant contributions to the field of protein detection, holding a total of 6 patents. His innovative approaches have advanced the methodologies used in biological research and diagnostics.
Latest Patents
One of his latest patents is a method for highly sensitive detection of protein-protein interactions. This invention provides an assay system using split luciferase, which boasts remarkably high detection sensitivity. In this embodiment, the binding of mutually interacting first and second proteins is detected by preparing a first fusion protein that comprises the first protein fused with a specific peptide. Additionally, a second fusion protein is created, which includes the second protein fused with a peptide selected from a specific group. The complex formed by the binding of these proteins allows for the detection of luminescence emitted, enhancing the sensitivity of protein interaction assays.
Another notable patent is an expression vector designed for establishing highly productive cells. This invention offers an effective expression vector that facilitates the efficient establishment of transformed cells, which express the targeted protein gene at high levels. The vector includes a cassette for expressing a drug-selective marker gene containing an mRNA destabilizing sequence, along with elements that stabilize gene expression. The mRNA destabilizing sequence is derived from an AT-rich sequence found in the 3'-untranslated region of various genes, while the stabilizing element is sourced from the Chinese hamster genome.
Career Highlights
Shigeaki Nishii has worked with notable organizations, including Toyo Boseki Kabushiki Kaisha and The University of Tokyo. His work in these institutions has allowed him to develop and refine his innovative technologies, contributing to advancements in the field of biotechnology.
Collaborations
Throughout his career, Nishii has collaborated with esteemed colleagues such as Kenji Masuda and Kazuhiro Matsui. These partnerships have fostered a collaborative environment that has led to significant advancements in their respective fields.
Conclusion
Shigeaki Nishii's contributions to protein detection technologies exemplify the impact of innovative thinking in scientific research. His patents reflect a commitment to enhancing the sensitivity and efficiency of biological assays, paving the way for future advancements in the field.