The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 08, 2013
Filed:
Jun. 04, 2010
Tomomi Yamazaki, Tsuruga, JP;
Kenji Masuda, Tsuruga, JP;
Shigeaki Nishii, Tsuruga, JP;
Bunsei Kawakami, Tsuruga, JP;
Tomomi Yamazaki, Tsuruga, JP;
Kenji Masuda, Tsuruga, JP;
Shigeaki Nishii, Tsuruga, JP;
Bunsei Kawakami, Tsuruga, JP;
Toyo Boseki Kabushiki Kaisha, Osaka-shi, JP;
Abstract
The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3'-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.