The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2017
Filed:
Jan. 31, 2014
The University of Tokyo, Tokyo, JP;
Probex Inc., Tokyo, JP;
Toyo Boseki Kabushiki Kaisha, Osaka, JP;
Takeaki Ozawa, Tokyo, JP;
Naomi Misawa, Tokyo, JP;
Kenji Miura, Tokyo, JP;
Tasuku Okamoto, Tokyo, JP;
Shigeaki Nishii, Tokyo, JP;
Kenji Masuda, Tokyo, JP;
The University of Tokyo, Tokyo, JP;
Toyo Boseki Kabushiki Kaisha, Osaka, JP;
ProbeX Inc., Tokyo, JP;
Abstract
The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.