Mie Goto

Title: Mie Goto: Innovator in Cell-Free Protein Synthesis

Introduction

Mie Goto is a prominent inventor based in Kanagawa, Japan. He has made significant contributions to the field of protein synthesis, particularly in developing methods for producing membrane proteins. With a total of 2 patents, Goto's work is paving the way for advancements in biotechnology.

Latest Patents

Mie Goto's latest patents include innovative methods for utilizing cell-free protein synthesis to produce membrane proteins. One of his patents describes a method for producing a membrane protein folded to its native or active structure within a lipid disc or liposome. This method involves preparing a reaction solution containing a polynucleotide encoding the membrane protein, a steroidal detergent, and a phospholipid. The process includes decreasing the concentration of the steroidal detergent and synthesizing the membrane protein while forming a lipid disk or liposome.

Another patent focuses on producing a thioredoxin-fused membrane protein using a cell-free protein synthesis system. This method allows for the expression of highly hydrophobic proteins, such as G protein-coupled receptors, in a soluble state that can form a biologically active three-dimensional structure. Goto's inventions provide a recombinant polynucleotide encoding the thioredoxin-fused membrane protein, enhancing the efficiency of protein synthesis.

Career Highlights

Mie Goto is associated with Riken Corporation, a leading research institution in Japan. His work at Riken has allowed him to explore innovative approaches to protein synthesis, contributing to the advancement of biotechnological applications.

Collaborations

Mie Goto has collaborated with notable coworkers, including Mikako Shirouzu and Shigeyuki Yokoyama. These collaborations have further enriched his research and development efforts in the field of protein synthesis.

Conclusion

Mie Goto's contributions to cell-free protein synthesis represent a significant advancement in biotechnology. His innovative methods for producing membrane proteins are essential for future research and applications in the field.