The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 07, 2007
Filed:
Nov. 19, 2004
Mikako Shirouzu, Yokohama, JP;
Goushi Ishihara, Yokohama, JP;
Mihoro Saeki, Yokohama, JP;
Mie Goto, Yokohama, JP;
Kaori Tajima, Yokohama, JP;
Takanori Kigawa, Yokohama, JP;
Shigeyuki Yokoyama, Yokohama, JP;
Mikako Shirouzu, Yokohama, JP;
Goushi Ishihara, Yokohama, JP;
Mihoro Saeki, Yokohama, JP;
Mie Goto, Yokohama, JP;
Kaori Tajima, Yokohama, JP;
Takanori Kigawa, Yokohama, JP;
Shigeyuki Yokoyama, Yokohama, JP;
Riken, Wako-Shi, Saitama, JP;
Abstract
We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.