The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 24, 2012
Filed:
Nov. 05, 2008
Shigeyuki Yokoyama, Kanagawa, JP;
Kazumi Shimono, Kanagawa, JP;
Mikako Shirouzu, Kanagawa, JP;
Mie Goto, Kanagawa, JP;
Shigeyuki Yokoyama, Kanagawa, JP;
Kazumi Shimono, Kanagawa, JP;
Mikako Shirouzu, Kanagawa, JP;
Mie Goto, Kanagawa, JP;
Riken, Wako-shi, Saitama, JP;
Abstract
A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.