Company Filing History:
Years Active: 2005-2011
Title: **Innovative Contributions of Michael Levene in Nucleic Acid Sequencing**
Introduction
Michael Levene is an esteemed inventor based in Ithaca, NY, who has made significant strides in the field of nucleic acid sequencing. With an impressive portfolio of 13 patents, Levene's work focuses on developing methods that enhance the efficiency and accuracy of sequencing target nucleic acid molecules. His inventions exemplify the intersection of technology and biology, paving the way for advancements in genetic research and applications.
Latest Patents
Levene's latest patents introduce groundbreaking methods in nucleic acid sequencing. One notable patent is directed to a method of sequencing a target nucleic acid molecule with a plurality of bases. This invention measures the temporal order of base additions during the polymerization reaction, using a novel approach involving labeled nucleotides. Specifically, each labeled nucleotide has an acceptor fluorophore attached to its phosphate portion, which is removed upon incorporation into a growing strand. This allows for the fluorescent signal to be emitted through a resonance energy transfer between donor and acceptor fluorophores, leading to precise identification of the incorporated bases.
Another of his significant inventions outlines a method for analyzing nucleic acid sequences, which involves creating a complex of a polymerase enzyme, target nucleic acid, and a primer immobilized on a support. The fluorescent label connected to the terminal phosphate group of the nucleotide aids in monitoring the extension of the growing nucleic acid strand. This method distinguishes between incorporated nucleotide analogs and freely diffusing labels based on their retention times within the observation volume, enhancing the efficiency of sequencing.
Career Highlights
Michael Levene has made remarkable contributions to his field as a key inventor at Cornell Research Foundation Inc. His dedication to pushing the boundaries of nucleic acid research has positioned him as a leading figure in innovative sequencing techniques. His work not only advances scientific understanding but also has practical implications for industries such as biotechnology and personalized medicine.
Collaborations
Throughout his career, Levene has worked alongside esteemed colleagues, including Jonas Korlach and Watt W. Webb. These collaborations have fostered a collective effort towards revolutionizing the field of nucleic acid sequencing, combining their expertise to drive innovative solutions.
Conclusion
Michael Levene's innovations in nucleic acid sequencing demonstrate the vital role of inventors in advancing scientific understanding and technology. His commitment to developing new methodologies has opened avenues for further research and applications in genetics, marking him as a pivotal figure in the ongoing quest to decode the complexities of life at the molecular level.