The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 17, 2011
Filed:
Jan. 19, 2006
Jonas Korlach, Ithaca, NY (US);
Watt W. Webb, Ithaca, NY (US);
Michael Levene, Ithaca, NY (US);
Stephen Turner, Ithaca, NY (US);
Harold G. Craighead, Ithaca, NY (US);
Mathieu Foquet, Ithaca, NY (US);
Jonas Korlach, Ithaca, NY (US);
Watt W. Webb, Ithaca, NY (US);
Michael Levene, Ithaca, NY (US);
Stephen Turner, Ithaca, NY (US);
Harold G. Craighead, Ithaca, NY (US);
Mathieu Foquet, Ithaca, NY (US);
Cornell Research Foundation, Ithaca, NY (US);
Abstract
The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.