Mark Danielsen

Title: Mark Danielsen: Innovator in Polynucleotide Detection

Introduction

Mark Danielsen is a notable inventor based in Germantown, MD (US). He has made significant contributions to the field of molecular biology, particularly in the detection of target polynucleotides. With a total of 4 patents to his name, Danielsen's work has advanced the methodologies used in genetic research and diagnostics.

Latest Patents

Danielsen's latest patents include innovative methods for detecting target polynucleotides. One of his notable inventions is the Exonuclease Cycling Assay. This method involves hybridizing a target polynucleotide with a probe to form a hybrid. The hybrid is then exposed to a 5' exonuclease, which digests the probe and dissociates the target polynucleotide. By repeating the hybridization and digestion steps, the presence of the target polynucleotide can be detected through the digested probes.

Another significant patent is the Polymerase Driven NESA. This method begins with exposing the target polynucleotide to an initiating oligonucleotide. The oligonucleotide is then extended and ligated to form a circular oligonucleotide with a nicking endonuclease (NE) recognition sequence. Following this, the circular oligonucleotide is exposed to a DNA polymerase to synthesize DNA with an NE recognition sequence. The synthesized DNA is then used to form a double-stranded DNA, which is cleaved by a nicking endonuclease. The presence of the cleaved probe indicates the presence of the target polynucleotide.

Career Highlights

Mark Danielsen has established himself as a key figure in his field through his innovative research and patent contributions. His work at Georgetown University has allowed him to collaborate with other experts and further his research endeavors.

Collaborations

Danielsen has worked alongside talented colleagues, including Berenice Alfonso and Bolor Tumurpurev. Their collaborative efforts have contributed to the advancement of molecular detection techniques.

Conclusion

Mark Danielsen's contributions to the field of molecular biology through his patents and research at Georgetown University highlight his role as an innovator in polynucleotide detection. His work continues to influence the methodologies used in genetic research and diagnostics.