The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 21, 2015
Filed:
Feb. 15, 2006
Mark Danielsen, Germantown, MD (US);
Eugene A. Davidson, Boynton Beach, FL (US);
Kenneth L. Dretchen, North Potomac, MD (US);
Traci K. Pals, Alexandria, VA (US);
Mark Danielsen, Germantown, MD (US);
Eugene A. Davidson, Boynton Beach, FL (US);
Kenneth L. Dretchen, North Potomac, MD (US);
Traci K. Pals, Alexandria, VA (US);
Georgetown University, Washington, DC (US);
Abstract
A method for detecting the presence of a target nucleotide sequence in a sample of DNA is described herein in which a test sample comprising single stranded DNA is exposed to a DNA probe and a nicking endonuclease under conditions that would permit sequence-specific hybridization of the probe to a complementary target sequence. The probe comprises a sequence complementary to the target sequence to be detected and this sequence also includes a recognition sequence for the nicking endonuclease. If the sample contains the target sequence, the probe hybridizes to the target and is cleaved by the nicking endonuclease, which leaves the target intact. Observing the presence of probe cleaved by the nicking endonuclease indicates the presence of the target nucleotide sequence in the sample of DNA.