The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 02, 2019
Filed:
Mar. 14, 2014
Applicant:
Georgetown University, Washington, DC (US);
Inventors:
Mark Danielsen, Germantown, MD (US);
Berenice Alfonso, Fairfax, VA (US);
Bolor Tumurpurev, Washington, DC (US);
Assignee:
GEORGETOWN UNIVERSITY, Washington, DC (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6823 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6823 (2013.01); C12Q 1/6823 (2013.01); C12Q 2521/319 (2013.01); C12Q 2525/125 (2013.01);
Abstract
The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising hybridizing the target polynucleotide with a probe to form a hybrid; exposing the hybrid to a 5' exonuclease so that the probe in the hybrid is digested and the target polynucleotide is dissociated from the digested probe; repeating the hybridization step and the digestion step; and detecting the digested probes. The presence of the digested probes indicates the presence of the target polynucleotide.