Kevin M O'Brien

Title: Kevin M O'Brien: Innovator in PCR and HLA Typing Technologies

Introduction

Kevin M O'Brien is a notable inventor based in Sahuarita, AZ (US). He has made significant contributions to the field of genetic testing through his innovative methods for PCR and HLA typing. With a total of 3 patents, O'Brien's work has advanced the capabilities of genetic analysis, particularly in utilizing unpurified samples.

Latest Patents

O'Brien's latest patents focus on methods for amplifying genes or RNA using a tandem PCR process. These methods are designed to work with raw, unpurified samples such as mouthwash, cheek swabs, and ORAGENE-stabilized saliva. The first PCR reaction employs locus-specific primers, while the second PCR reaction utilizes primers that are specific for a sub-sequence of the locus-specific primers. This innovative approach allows for effective RNA amplification, where the first PCR serves as reverse transcription, providing cDNA templates for further analysis. The resulting amplicons can be used for complex, microarray-based genetic testing, showcasing O'Brien's commitment to enhancing genetic research methodologies.

Career Highlights

Throughout his career, Kevin M O'Brien has worked with various organizations, including Genomics USA, Inc. His experience in the field has allowed him to develop cutting-edge technologies that have practical applications in genetic testing and analysis.

Collaborations

O'Brien has collaborated with notable professionals in the field, including Michael E Hogan and Georgina Lopez Padilla. These partnerships have contributed to the advancement of his research and the successful implementation of his patented methods.

Conclusion

Kevin M O'Brien's innovative work in PCR and HLA typing has significantly impacted the field of genetic testing. His patents reflect a deep understanding of genetic analysis and a commitment to improving methodologies in this vital area of research.