The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 08, 2014
Filed:
Sep. 24, 2010
Michael E. Hogan, Tuscan, AZ (US);
Georgina Lopez Padilla, Tucson, AZ (US);
Melissa R. May, Tucson, AZ (US);
Andrew T. Abalos, Tucson, AZ (US);
Frederick H. Eggars, Sahuarita, AZ (US);
Kevin M. O'brien, Sahuarita, AZ (US);
Michael E. Hogan, Tuscan, AZ (US);
Georgina Lopez Padilla, Tucson, AZ (US);
Melissa R. May, Tucson, AZ (US);
Andrew T. Abalos, Tucson, AZ (US);
Frederick H. Eggars, Sahuarita, AZ (US);
Kevin M. O'Brien, Sahuarita, AZ (US);
Genomics USA, Inc., Inverness, IL (US);
Abstract
Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the secondary PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample, hybridizing the resulting amplicon or sets thereof to probes with sequences of gene-associated allele variations. A detectable signal indicating hybridization corresponds to an allelotype of the gene or a set of allelotypes for the set of genes.