The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 16, 2016
Filed:
Oct. 12, 2011
Michael E. Hogan, Tuscan, AZ (US);
Georgina Lopez Padilla, Tucson, AZ (US);
Melissa R. May, Tucson, AZ (US);
Andrew T. Abalos, Tucson, AZ (US);
Frederick H. Eggars, Sahuarita, AZ (US);
Kevin M. O'brien, Sahuarita, AZ (US);
Michael E. Hogan, Tuscan, AZ (US);
Georgina Lopez Padilla, Tucson, AZ (US);
Melissa R. May, Tucson, AZ (US);
Andrew T. Abalos, Tucson, AZ (US);
Frederick H. Eggars, Sahuarita, AZ (US);
Kevin M. O'Brien, Sahuarita, AZ (US);
Other;
Abstract
Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE™-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample.