Farren J Isaacs

Title: Farren J Isaacs: Innovator in Genome Engineering

Introduction

Farren J Isaacs is a prominent inventor based in Stamford, CT (US). He has made significant contributions to the field of genome engineering, holding a total of 6 patents. His work focuses on advancing gene editing techniques that have the potential to revolutionize genetic research and applications.

Latest Patents

One of his latest patents is titled "Multiplex genome engineering in eukaryotes." This invention provides compositions and methods for gene editing that utilize an oligo-based annealing mechanism rooted in DNA replication rather than homologous recombination (HR). The methods demonstrate oligo incorporation efficiencies that are comparable to, and often exceed, those of CRISPR/Cas9 editing, all without the need for double strand breaks (DSBs). By relying on multiplex annealing of oligos, the process is highly scalable across genomic regions of interest, allowing for the generation of multiple scarless modifications of a chromosome simultaneously. This innovation enables the creation of combinatorial genomic diversity across a population of cells in a single transformation event, facilitating genome-wide changes that can be massively parallelized and amplified through systematic strain mating.

Another notable patent is "Phosphopeptide-encoding oligonucleotide libraries and methods for detecting phosphorylation-dependent molecular interactions." This invention relates to libraries of phosphopeptide-encoding oligonucleotides and methods for preparing such libraries. It also includes methods for detecting, visualizing, or screening for phosphorylation-dependent protein-protein interactions using recombinant phosphopeptides and/or phosphopeptide-encoding oligonucleotides. Additionally, it encompasses sets or kits of oligonucleotides that encode phosphopeptides.

Career Highlights

Farren J Isaacs has worked with esteemed institutions such as Yale University and Agilent Technologies, Inc. His career reflects a commitment to advancing the field of genetic engineering through innovative research and development.

Collaborations

Throughout his career, Farren has collaborated with notable individuals, including Miriam Amiram and Adrian Haimovich. These collaborations have contributed to the success of his research and the development of his patents.

Conclusion

Farren J Isaacs is a leading figure in the realm of genome engineering, with a focus on innovative gene editing techniques. His contributions have the potential to significantly impact genetic research and applications in various fields.