The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 09, 2022
Filed:
Sep. 28, 2015
Applicant:
Yale University, New Haven, CT (US);
Inventors:
Farren J. Isaacs, Stamford, CT (US);
Ryan Gallagher, New Haven, CT (US);
Jaymin Patel, New Haven, CT (US);
Alexis Rovner, Cambridge, MA (US);
Assignee:
YALE UNIVERSITY, New Haven, CT (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/70 (2006.01); C12N 15/79 (2006.01); C12N 15/74 (2006.01); C12N 1/36 (2006.01); C12P 1/00 (2006.01); C12N 15/10 (2006.01); C12N 1/20 (2006.01); C12P 21/02 (2006.01); C12P 1/04 (2006.01); C12R 1/19 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12N 1/36 (2013.01); C12N 15/102 (2013.01); C12N 15/74 (2013.01); C12N 15/79 (2013.01); C12P 1/00 (2013.01); C12P 1/04 (2013.01); C12P 21/02 (2013.01); C12R 2001/19 (2021.05);
Abstract
Methods and strategies for introducing genetic safeguards into microorganisms, genetically modified organisms (GMO) including the safeguards, and methods of use thereof are provided. The genetic safeguards generally impart a low escape frequency, are robust, and are modular. Safeguards with low escape frequency prevent the rise of mutants escaping defined media and limit growth in the wild. Robust safeguards retain wild-type levels of fitness while also maintaining containment in diverse growth conditions.