Location History:
- Rockville, MD (US) (2008 - 2019)
- Bethesda, MD (US) (2024)
Company Filing History:
5 out of 2,123 
3 out of 3,540 
1 out of 9 Years Active: 2008-2024
Title: The Innovative Contributions of Daniel H. Appella
Introduction
Daniel H. Appella is a notable inventor based in Rockville, MD (US). He has made significant contributions to the field of biological sample analysis, holding a total of 8 patents. His work focuses on innovative methods for target enrichment and detection of nucleic acids, which have important implications in various scientific and medical applications.
Latest Patents
One of his latest patents involves a method for RNA target enrichment or depletion from biological samples. This method utilizes a probe that includes a left-handed PNA pair and a targeting moiety. The left-handed PNA pair consists of complementary PNAs that are chiral and feature a cyclic backbone modification, resulting in a left-handed helical structure. The capture surface incorporates the other half of the left-handed PNA pair, allowing for hybridization of the probe to the capture surface, which may be a magnetic bead.
Another significant patent by Appella concerns cyclopentane-peptide nucleic acids for the qualitative and quantitative detection of nucleic acids. This invention outlines methods for detecting a nucleic acid of interest in a solution. The process involves contacting a solution suspected of containing the nucleic acid with a PNA capture probe and a PNA reporter probe. The PNA capture probe is designed with at least two trans-cyclopentanes, while the PNA reporter probe includes at least six biotin groups. The detection occurs when 1-1000 molecules of the nucleic acid of interest are present in the tested solution.
Career Highlights
Daniel H. Appella has worked with prestigious institutions such as Northwestern University and the National Institutes of Health, which is a component of the US Department of Health & Human Services. His experience in these organizations has allowed him to contribute to groundbreaking research and development in the field of biological sciences.
Collaborations
Throughout his career, Appella has collaborated with esteemed colleagues, including Sui Huang and John Thomas Norton. These partnerships have further enhanced his research and innovation efforts.
Conclusion
Daniel H. Appella's contributions to the field of biological sample analysis through his innovative patents and collaborations highlight his role as a significant inventor. His work continues to influence advancements in nucleic acid detection and enrichment methods.