Northwestern University

The United States of America, as Represented by the Secretary, Department of Health and Human Services

Altratech Limited

Daniel H Appella

Sui Huang

John Thomas Norton

Christopher Micklitsch

Mark Witschi

Michael C Myers

Brian O'Farrell

Bereket Yemane

Chao Zhao

Mark Witschl

Claire O'Connell

Kaylyn Oshaben

Michael T Scerba

Herman Nikolayevskiy

A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.

RNA target enrichment or depletion of biological samples

The invention concerns methods for detecting a nucleic acid of interest in a solution comprising (a) contacting a solution suspected of containing the nucleic acid of interest with a PNA capture probe and a PNA reporter probe; wherein (i) the PNA capture probe comprises at least two trans-cyclopentanes; (ii) the PNA reporter probe comprises at least six biotin groups; (iii) the PNA capture probe bound to a surface; and (iv) the PNA capture probe and the PNA reporter probe each comprise a nucleobase sequence that is complementary to different non-overlapping portions of the nucleic acid of interest; (b) detecting the presence of the PNA capture probe and the PNA reporter probe bound to the surface; wherein the nucleic acid of interest is detected when 1-1000 molecules of the nucleic acid of interest are present in the solution being tested.

Cyclopentane-peptide nucleic acids for qualitative and quantitative detection of nucleic acids

The present invention relates to anti-cancer compounds, methods for their discovery, and their therapeutic use. In particular, the present invention provides analogs of the known anti-cancer compound amonafide, and structurally and functionally related compounds, and methods of using such compounds as therapeutic agents to treat a number of conditions associated with hyperproliferation.

Anti-cancer compounds

Disclosed is a method for lowering the detection limit in a method of detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA with a substrate having a second PNA affixed thereto, the second PNA comprising at least one trans-cyclopentane residue, wherein the first PNA has two linker-attached biotins attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. Also disclosed are a detection assay and a kit for detecting a target nucleic acid.

Cross-coupled peptide nucleic acids for detection of nucleic acids of pathogens

Ketoamine compounds and related methods for reactivation of tumor suppressor protein p53.

Compounds and related methods for mutant p53 reactivation

Growing community of inventors

Rockville, MD, United States of America

Daniel H Appella