Chudi Guan

Title: Chudi Guan: Innovator in DNA Fragmentation Techniques

Introduction

Chudi Guan is a prominent inventor based in Wenham, MA (US), known for his significant contributions to the field of molecular biology. With a total of 12 patents to his name, he has made remarkable advancements in DNA fragmentation methods.

Latest Patents

One of Chudi Guan's latest patents is a method for fragmenting DNA by nick translation. This innovative approach provides kits, compositions, and methods that relate to DNA fragmentation. An embodiment of this composition combines one or more enzymes capable of nick translating activity, a dNTP mix comprising at least one dNTP with a modified base, and at least one modification-sensitive nicking endonuclease. This endonuclease is prevented from nicking DNA if its recognition site contains the modified base. When this composition is added to a sample with a double-stranded DNA template that has recognition sites for the nicking endonuclease, a reaction mix is produced. This mix can be incubated for any time period exceeding about 5 minutes to generate fragments of a desired size of the double-stranded DNA template. The fragments produced include the modified base, ensuring they are not re-nicked by the nicking endonuclease.

Career Highlights

Chudi Guan has worked with notable organizations such as New England Biolabs, Inc. and Temple University. His experience in these institutions has contributed to his expertise in the field of molecular biology and DNA research.

Collaborations

Chudi has collaborated with esteemed colleagues, including Ellen P Guthrie and David Landry, further enhancing his work in DNA fragmentation techniques.

Conclusion

Chudi Guan's innovative methods in DNA fragmentation have made a significant impact in molecular biology. His contributions continue to advance the understanding and application of DNA research.