The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 20, 2010
Filed:
Oct. 20, 2005
Thomas C. Evans, Topsfield, MA (US);
Barton Slatko, Ipswich, MA (US);
Lixin Chen, Beverly, MA (US);
Romaldas Vaisvila, Ipswich, MA (US);
Chudi Guan, Wenham, MA (US);
Thomas C. Evans, Topsfield, MA (US);
Barton Slatko, Ipswich, MA (US);
Lixin Chen, Beverly, MA (US);
Romaldas Vaisvila, Ipswich, MA (US);
Chudi Guan, Wenham, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Methods and compositions are provided for repairing a polynucleotide so that it can be synthesized efficiently with improved fidelity and yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. These enzymes are optionally selected according to their ability to withstand high temperatures so they can be included in an amplification mixture. The reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture.