The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 22, 2014
Filed:
Feb. 03, 2010
Applicants:
Pei-chung Hsieh, Topsfield, MA (US);
Chudi Guan, Wenham, MA (US);
Inventors:
Pei-Chung Hsieh, Topsfield, MA (US);
Chudi Guan, Wenham, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/16 (2006.01); C12N 15/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/34 (2006.01); C12P 19/30 (2006.01); C07H 21/04 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract
An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.