The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2015

Filed:

Aug. 25, 2010
Applicants:

Jurate Bitinaite, Rowley, MA (US);

Romualdas Vaisvila, Ipswich, MA (US);

Sriharsa Pradhan, Wenham, MA (US);

Yu Zheng, Topsfield, MA (US);

Richard J. Roberts, Wenham, MA (US);

Devora Cohen-karni, Cambridge, MA (US);

Christopher Noren, Boxford, MA (US);

Elisabeth A. Raleigh, Somerville, MA (US);

Geoffrey Wilson, South Hamilton, MA (US);

Hang-gyeong Chin, South Hamilton, MA (US);

Inventors:

Jurate Bitinaite, Rowley, MA (US);

Romualdas Vaisvila, Ipswich, MA (US);

Sriharsa Pradhan, Wenham, MA (US);

Yu Zheng, Topsfield, MA (US);

Richard J. Roberts, Wenham, MA (US);

Devora Cohen-Karni, Cambridge, MA (US);

Christopher Noren, Boxford, MA (US);

Elisabeth A. Raleigh, Somerville, MA (US);

Geoffrey Wilson, South Hamilton, MA (US);

Hang-Gyeong Chin, South Hamilton, MA (US);

Assignee:

New England Biolabs, Inc., Ipswich, MA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01); C12Q 1/6827 (2013.01); C12Q 2521/331 (2013.01); C12Q 2525/191 (2013.01); C12Q 2531/113 (2013.01);
Abstract

Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.


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