The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 07, 2010
Filed:
Feb. 10, 2006
Toomas Silla, Tartu, EE;
Ingrid Tagen, Tartu, EE;
Jelizaveta Geimanen, Tartu, EE;
Kadri Janikson, Tartu, EE;
Aare Abroi, Tartu, EE;
Ene Ustav, Tartu, EE;
Mart Ustav, Tartu, EE;
Tiiu Mandel, Tartu, EE;
Toomas Silla, Tartu, EE;
Ingrid Tagen, Tartu, EE;
Jelizaveta Geimanen, Tartu, EE;
Kadri Janikson, Tartu, EE;
Aare Abroi, Tartu, EE;
Ene Ustav, Tartu, EE;
Mart Ustav, Tartu, EE;
Tiiu Mandel, Tartu, EE;
Kosagen Cell Factory Oü, Tartu, EE;
Abstract
This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost with the frequency of 6% per generation. Significantly long stable maintenance replication can also be provided without selection pressure. We also demonstrate that PyV core origin maintenance function/replication activation could be provided by Epstein-Barr virus Family of repeats and EBNA1 protein. The maintenance of the Polyomavirus core origin plasmid was characterized by 13% loss of the plasmid during one cell generation in the case of EBV FR harboring plasmids. Our data clearly indicate that maintenance functions from different viruses can provide segregation/partitioning function to different heterologous origins in variety of cells and can be used in expression of gene products.