The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 18, 1997

Filed:

Dec. 20, 1994
Applicant:
Inventors:

Yoshio Furutani, Miuragun, JP;

Hiroaki Shimada, Mobara, JP;

Izumi Mita, Mobara, JP;

Kazuaki Manabe, Mobara, JP;

Masaru Honjo, Mobara, JP;

Noboru Tomioka, Mobara, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12P / ;
U.S. Cl.
CPC ...
435 691 ; 4352523 ; 43525231 ; 4353201 ;
Abstract

A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.


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