The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 22, 1976
Filed:
Oct. 24, 1974
Bela Johan, Budapest, HU;
Laszlo Szemler, Budapest, HU;
Janos Fulop, Budapest, HU;
Tamas Szontagh, Budapest, HU;
Emilia Simonovits nee Czink, Budapest, HU;
Judit Bekes nee Erdos, Budapest, HU;
Laszlo Kuti, Budapest, HU;
Robert Toros, Budapest, HU;
Denes Szekely, Budapest, HU;
Laszlo Szabo, Budapest, HU;
Karoly Seitz, Budapest, HU;
Tade Vajda, Budapest, HU;
Erzsebet Kovacs nee Komoroczy, Budapest, HU;
Aniko Hargital nee Franyo, Budapest, HU;
Karoly Polinszky, Budapest, HU;
Richter Gedeon Vegyeszeti Gyar Rt., Budapest, HU;
Abstract
The invention relates to a method for increasing the vitamin B.sub.12 production of fermentation processes performed in a known way with a mixed population of mesophilic methane-producing bacteria under anaerobic conditions. According to the invention in an enrichment period of preferably 4 to 7 days a nutrient concentrate containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15 is added in daily portions to a fermentation broth obtained from a usual vitamin B.sub.12 fermentation process and containing living bacterium populations so as to increase the total concentration of assimilable nitrogen by a factor of maximum 4 until the end of the enrichment period, and thereafter the fermentation is terminated and the obtained fermentation broth with an increased vitamin B.sub.12 content is processed, or the fermentation is continued by periodically removing a portion of the fermentation broth and supplementing it with a same volume of fresh nutrient medium containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15. By this process fermentation broths with as high vitamin B.sub.12 concentration as 40.000 mcg./l. can be obtained.