The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 05, 2025
Filed:
Jan. 21, 2021
Zhejiang Yongtai Technology Co., Ltd., Zhejiang, CN;
Zhejiang University of Technology, Zhejiang, CN;
Zhejiang Yongtai Pharmaceutical Co., Ltd., Zhejiang, CN;
Zhiqiang Liu, Zhejiang, CN;
Feng Cheng, Zhejiang, CN;
Xiaojian Zhang, Zhejiang, CN;
Dongxu Jia, Zhejiang, CN;
Yuguo Zheng, Zhejiang, CN;
Renbao He, Zhejiang, CN;
Yizhong Jin, Zhejiang, CN;
Hongming Shao, Zhejiang, CN;
Jiaohua Lin, Zhejiang, CN;
Feng Zhang, Zhejiang, CN;
ZHEJIANG YONGTAI TECHNOLOGY CO., LTD., Zhejiang, CN;
ZHEJIANG UNIVERSITY OF TECHNOLOGY, Zhejiang, CN;
ZHEJIANG YONGTAI PHARMACEUTICAL CO., LTD., Zhejiang, CN;
Abstract
The present invention discloses a transaminase mutant and application thereof in preparation of sitagliptin intermediates, the transaminase mutant is obtained by substitution of tyrosine with proline at position 74, substitution of glutamic acid with aspartic acid at position 228, substitution of leucine with alanine at position 254 and substitution of methionine with threonine at position 290 of the amino acid sequence shown in SEQ ID NO: 2. The present invention uses wet cells or a purified transaminase as a biocatalyst and a sitagliptin precursor ketone or a prochiral carbonyl compound as a substrate to prepare a sitagliptin intermediate or a sitagliptin ester intermediate; the total yield of the method reaches about 82%, and e.e. value of the product reaches 99%.