The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2025
Filed:
Dec. 27, 2022
Hybridoma cell line of secreting cyproheptadine monoclonal antibodies and preparation method thereof
Jiangnan University, Wuxi, CN;
Chuanlai Xu, Wuxi, CN;
Hua Kuang, Wuxi, CN;
Wei Jiang, Wuxi, CN;
Liguang Xu, Wuxi, CN;
Wei Ma, Wuxi, CN;
Liqiang Liu, Wuxi, CN;
Xiaoling Wu, Wuxi, CN;
Shanshan Song, Wuxi, CN;
Yongming Hu, Wuxi, CN;
JIANGNAN UNIVERSITY, Jiangsu, CN;
Abstract
A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.