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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10752963 B1

PDFFull Text
Date of Patent:
Aug. 25, 2020

Filed:

Jul. 24, 2018

Micro-fluidic chip and its modification method and application in detection of the quantity of food bacteria

Applicant:

Greentown Agricultural Testing Technology Co., Ltd, Hangzhou, Zhejiang Province, CN;

Inventors:

Xingkai Hao, Hangzhou, CN;

Yuqian Jiang, Ottawa, CA;

Xudong Cao, Ottawa, CA;

Chuanpi Wang, Hangzhou, CN;

Min Zhou, Hangzhou, CN;

Meina Sun, Hangzhou, CN;

Zhen Wang, Hangzhou, CN;

Assignee:

GREENTOWN AGRICULTURAL TESTING TECHNOLOGY CO., LTD., Hangzhou, CN;

Attorneys:

Jiwen Chen

Jacobson Holman PLLC

Primary Examiner:

Joseph G Dauner

Int. Cl.
CPC ...
C12Q 1/689 (2018.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/689 (2013.01); B01L 3/502707 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/123 (2013.01); C12Q 2600/142 (2013.01);
Abstract

A micro-fluidic chip and its modification method and application in detection of food bacteria quality, includes the following steps: changing a functional group —CHon the internal surface of micro-fluidic channel of micro-fluidic chip to a functional group —OH through modification; Supplying amino silane reagent to the micro-fluidic channel; supplying dendritic polymer as modified by —COOH to internal surface of the micro-fluidic channel after drying; grafting primer of aminated aptamer RCA on terminus 5' and hybrid from its padlock probe on dendrimer on internal surface of the micro-fluidic channel; wherein, the padlock probe is the complementary sequence of the aptamer of the target pathogenic bacteria to be tested; after that, supplying RCA reaction reagent to the micro-fluidic channel to make aptamer RCA generate long-chain aptamer in series. The present invention adopts two RCA reactions of varied functions in combination, and uses dendritic polymer to modify internal surface of the chip.

Find Patent Forward Citations

Hao, Xingkai. (2020). Micro-fluidic chip and its modification method and application in detection of the quantity of food bacteria (U.S. Patent No. 10752963). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10752963

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