Company Filing History:
Years Active: 2018-2019
Title: Innovations of Xiaowei Yu in Biotechnology
Introduction
Xiaowei Yu is a prominent inventor based in Wuxi, China. He has made significant contributions to the field of biotechnology, particularly in the development of genetically engineered strains of yeast. With a total of 5 patents, his work focuses on enhancing the expression levels of foreign proteins, which has important implications for various biotechnological applications.
Latest Patents
One of Xiaowei Yu's latest patents is titled "High-secretion heat-resistant yeast genetically engineered strain and application thereof." This invention discloses a genetically engineered strain of yeast that is capable of expressing a lipase gene at a high secretion level under high-temperature conditions. The strain has been collected by the China Center for Type Culture Collection (CCTCC) with the collection number CCTCC NO: M 2016278. Sequencing analysis indicates that the lipase gene and its promoter sequence remain unmutated, suggesting that the high-secretion expression is due to mutations in other genomic sequences. Additionally, by employing a homologous double-crossover method to knock out the lipase gene, the constructed knockout strain can serve as an expression host for other exogenous proteins, enhancing their expression levels during high-temperature fermentation.
Another notable patent is the method for improving expression levels of foreign proteins through phospholipase fusion expression. This invention outlines a method that utilizes four proteins—PLA, MBP, CBD, and SUMO—as fusion tags to construct a fusion gene. The results show that the extracellular expression level and enzymatic activity of the fusion proteins are significantly increased compared to the original protein MOH. Notably, the fusion protein using PLA as the fusion tag exhibits the highest expression level, reaching up to 12 g·L in a 7 L fermentation tank during high-density fermentation.
Career Highlights
Xiaowei Yu is affiliated with Jiangnan University, where he continues to advance research in biotechnology. His innovative approaches and discoveries have positioned him as a key figure in the field, contributing to the development of more efficient biotechnological processes.
Collaborations
Xiaowei Yu collaborates with fellow researcher Yan Xu, enhancing the scope and impact of their research endeavors.
Conclusion
Xiaowei Yu's contributions to biotechnology through his innovative patents demonstrate his commitment to advancing the field. His work