Xiaorui Liu

Title: Innovations of Xiaorui Liu in Insulin and Liraglutide Preparation

Introduction

Xiaorui Liu is a prominent inventor based in Jiangsu, China. He has made significant contributions to the field of biopharmaceuticals, particularly in the preparation of insulin and liraglutide polypeptides. With a total of 2 patents to his name, Liu's work has the potential to impact diabetes treatment and management.

Latest Patents

Liu's latest patents include a novel pro-insulin aspart structure and a method for preparing insulin aspart. This innovative method involves designing the pro-insulin aspart sequence and constructing recombinant insulin aspart engineered bacteria. The process includes inducing the expression of an insulin fusion protein as an inclusion body in the engineered bacteria, followed by extraction through denaturing, renaturing enzymatic digestion, and purification operations. Notably, the method modifies the recombinant leading peptide and C-peptide sequences, eliminating the need for a cyanogen bromide cleavage step. Additionally, the C-peptide can be replaced by a single amino acid, which helps reduce quality loss during enzymatic digestion.

Another significant patent by Liu is the method for preparing a liraglutide intermediate polypeptide, specifically GLP-1 (7-37). This preparation method involves constructing recombinant liraglutide engineered bacteria to induce the expression of a liraglutide intermediate fusion protein in the form of inclusion bodies. The process includes denaturation, renaturation, enzyme digestion, separation, and purification to obtain the liraglutide intermediate polypeptide. By altering the expression pattern to produce intracellular insoluble inclusion bodies, Liu's method significantly increases the expression level, achieving a purity of over 87% and a yield exceeding 85%.

Career Highlights

Xiaorui Liu is currently employed at Amphastar Nanjing Pharmaceuticals Inc., where he continues to advance his research in biopharmaceuticals. His innovative approaches to polypeptide preparation have positioned him as a key figure in the industry.

Collaborations

Liu collaborates with notable colleagues, including Chuangen Tang and Shangshu Pan, who contribute to his research endeavors and innovations.

Conclusion

Xiaorui Liu's contributions to the field of biopharmaceuticals, particularly in insulin and liraglutide preparation, showcase his innovative spirit and dedication