The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 2025
Filed:
Sep. 12, 2018
Amphastar Nanjing Pharmaceuticals Inc, Jiangsu, CN;
Chuangen Tang, Jiangsu, CN;
Shangshu Pan, Jiangsu, CN;
Xiaorui Liu, Jiangsu, CN;
Cheng Li, Jiangsu, CN;
Huaiyan Cui, Jiangsu, CN;
Song Chen, Jiangsu, CN;
Haoning Zhang, Jiangsu, CN;
Jeffrey Ding, Jiangsu, CN;
AMPHASTAR NANJING PHARMACEUTICALS INC, Jiangsu, CN;
Abstract
Provided are a novel pro-insulin aspart structure design and a method for preparing the pro-insulin aspart. The method comprises designing the pro-insulin aspart sequence, constructing a recombinant insulin aspart engineered bacteria, inducing expression of an insulin fusion protein as an inclusion body in the engineered bacteria, and extracting the insulin fusion protein from the engineered bacteria by means of denaturing, renaturing enzymatic digestion, and separation purification operations. By modifying the recombinant leading peptide and C-peptide sequences, the method avoids the need to include a cyanogen bromide cleavage step. In some embodiments, the C-peptide is replaced by a single amino acid which tends to reduce quality loss associated with enzymatic digestion.