Villy Nielsen

Title: Villy Nielsen: Innovator in Mammalian Cell Culture

Introduction

Villy Nielsen is a notable inventor based in Roskilde, Denmark. He has made significant contributions to the field of mammalian cell culture, holding two patents that focus on innovative methods for cell attachment and cultivation.

Latest Patents

Nielsen's latest patents include a method for creating a surface of a vessel with polystyrene, nitrogen, and oxygen, which facilitates the attachment and cultivation of cells. This invention provides methods and compositions for cell attachment to, cultivation on, and detachment from a solid substrate surface. The substrate contains at least about 0.5% nitrogen, a sum of oxygen and nitrogen of greater than or equal to 17.2%, and a contact angle of at least about 13.9 degrees. Notably, this method does not require a feeder cell layer or an adlayer. In one embodiment, the cells are treated with a compound capable of inhibiting Rho kinase activity, while in another, they are treated with a compound capable of inhibiting Rho activity.

Career Highlights

Throughout his career, Villy Nielsen has worked with prominent companies such as Nunc A/S and Janssen Biotech, Inc. His experience in these organizations has contributed to his expertise in the field of cell culture and biotechnology.

Collaborations

Nielsen has collaborated with notable individuals in his field, including Benjamin Fryer and Shelley Nelson. These partnerships have likely enhanced his research and development efforts.

Conclusion

Villy Nielsen's innovative work in mammalian cell culture has led to significant advancements in the methods of cell attachment and cultivation. His contributions continue to impact the field positively.